At CytobodX, we offer advanced analytical services for the biophysical and functional characterization of recombinant and native proteins. Using a suite of state-of-the-art instruments, we help you assess stability, purity, folding, and molecular interactions with exceptional precision. Whether you’re validating a therapeutic candidate, optimizing protein formulation, or screening ligands, our expert team delivers actionable insights.

Available Technologies & Applications

1. Biolayer Interferometry (BLI) – Octet R8

Label-free, real-time analysis of biomolecular interactions

• Kinetics (kon, koff, KD) of antibody–antigen, protein–ligand, or protein–protein interactions
• High-throughput screening of binders (e.g., antibody panels)
• Epitope binning and competition assays

2. Microscale Thermophoresis (MST) – Monolith NT.115

Quantitative interaction studies in free solution

• KD determination in complex or unpurified samples
• Protein–ligand, protein–DNA, protein–ion interactions
• Requires minimal sample volume

3. Isothermal Titration Calorimetry (ITC) – MicroCal iTC200

Gold standard for thermodynamic profiling

• Accurate measurement of binding affinity, enthalpy (ΔH), entropy (ΔS), and stoichiometry
• Ideal for antibody–antigen, inhibitor–enzyme, and small molecule–protein interactions

4. SEC-MALS – Size Exclusion Chromatography with Multi-Angle Light Scattering

Molecular weight and oligomerization state in native conditions

• Determination of absolute molecular mass and aggregation
• Evaluation of protein monodispersity
• Characterization of protein complexes or conjugates (e.g., glycoproteins)

5. Nano Differential Scanning Fluorimetry (nanoDSF) – Tycho NT.6

Fast and precise assessment of protein stability

• Melting temperature (Tm), unfolding profiles, and formulation screening
• Ideal for buffer optimization and batch quality control

6. Dynamic Light Scattering (DLS) – Zetasizer Nano

Size distribution and aggregation detection

• Hydrodynamic radius and polydispersity index
• Aggregation monitoring and pre-crystallization screening
• Suitable for quality control of protein samples and nanoparticles

Applications & Use Cases

• Protein–ligand interaction profiling (KD, thermodynamics, specificity)
• Antibody–antigen binding and epitope characterization
• Protein formulation and buffer optimization
• Stability and aggregation assessment (pre-crystallization, therapeutic development)
• Batch-to-batch comparability and quality control

Deliverables

• Fully interpreted data reports (raw + processed)
• Binding curves, thermal profiles, SEC-MALS traces
• Key metrics (KD, Tm, MW, stoichiometry, etc.)
• Summary and recommendations by our scientists