We offer a specialized service for the production of recombinant extracellular vesicles (rEVs) engineered to express membrane proteins of interest in their native and functional conformation. This platform provides a powerful and physiologically relevant system for studying complex transmembrane proteins such as receptors, transporters, ion channels, or GPCRs in a functional and accessible format (examples of membrane proteins available).
What We Provide
Our service includes:
- Custom vector design and cell engineering for the expression of the target membrane protein
- Scalable production of rEVs from mammalian cell lines (HEK293)
- Purification of rEVs (100 nm average diameter)
- Characterization by:
- Western blotting
- Biolayer interferometry
- Electron microscopy
Applications
- Immunogen delivery for antibody or nanobody generation
- Functional assays involving membrane receptors or transporters
- Ligand screening and receptor–ligand interaction studies
- Target validation for therapeutic development
Why Choose Our Service?
- Native membrane environment preserving conformation and function
- Oriented surface display of extracellular domains
- Co-expression and surface engineering options available
- High-quality production and QC standards
- Scalable yield from microgram to milligram quantities
- Optional functional testing with your downstream application in mind
Workflow Summary
- Target protein evaluation and design strategy
- Expression vector construction
- Production and purification of rEVs
- Quality control and protein display validation
- Data report and delivery of purified rEVs
Project Timeline & Deliverables
- Typical turnaround time: 4–6 weeks
- Deliverables: Purified rEVs aliquoted and shipped in dry ice, data report
CytobodX 