Our service provides high-quality production of soluble recombinant proteins tailored for research applications. From gene design to purified protein, we offer a complete workflow optimized for yield, solubility, and biological functionality.

Service Overview

We offer end-to-end support for the expression and purification of recombinant proteins in soluble form:

• Gene Design & Vector Construction
  Codon optimization and subcloning into appropriate expression vectors (tags: His, GST, MBP, Fc, ..).
• Expression System Selection
  Choice of expression host based on protein characteristics and desired scale:
  • E. coli (fast, cost-effective)
  • Mammalian cells (HEK293, CHO – for complex folding/post-translational modifications)
  • Insect cells (Sf9/Baculovirus – balance between yield and folding)
• Optional Expression Optimization
  Screening of conditions (induction, temperature, time) to maximize soluble yield.
• Purification Workflow
  Multi-step purification pipeline adapted to the protein and application:
  • Affinity chromatography (Ni-NTA, Protein A/G, GST, etc.)
  • Size-exclusion chromatography (SEC)
  • Ion exchange or other polishing steps as needed
• Quality Control
  • SDS-PAGE
  • Concentration and purity measurement
  • Optional: endotoxin testing

Deliverables

• Soluble recombinant protein aliquoted and shipped in dry ice
• QC report (purity, concentration, yield, tag cleavage if applicable)